Description

ECOS™ Sonic™ Competent Cells allow you to clone and express in one novel strain. Also available with the pLysS plasmid, the Sonic™ strain is a BL21-derivative that features complete deletions of the endA and recA genes, eliminating degradation and recombination of DNA.

For optimal results, recommended for use in combination with Yeastern’s proprietary T&A™ expression vector, which will combine transformation, cloning, and protein expression into one single step.

Genotype

F^– ompT hsds_B (r_B^–m_B^–) gal dcm (DE3) ΔendA ΔrecA

Features

• Fast growth: Only 4-6hrs culture time required to isolates plasmid DNA
• Stable: Lack of ompT and Ion proteases to stabilize synthesized protein; deletion of endA and recA eliminates DNA degradation and recombination
• High efficiency and protein yield: 1 x 10⁸ cfu/ug
• Simple to use: clone and express with the same strain, no more sub-cloning required!

Applications

• protein expression
• preservation of the PCR product
• preservation of the expression plasmid
• plasmid quick production
• cell bank validation
• Antibody development and quick screening for the target proteins

Specifications

Species    E. coli

Strain       BL21(DE3) Derived

Efficiency   >1 x 10⁸ cfu/μg (FYE907-5VL / -20VL)

Format     Tube (100 μL) 

Quantity   5 x 100 μL  / 20 x 100 μL

Store at    -70°C.