Description

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).

Genotype

endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk^–, mk⁺) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ^– F^–

Features

Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.

Applications

• Cloning and subcloning
• Scale-up application
• Blue/white screening

Specifications

Species    E. coli

Strain       DH5α

Efficiency   >1 x 10⁸ cfu/μg (FYE607-10VL / -80VL)

Format     Tube (100 μL) 

Quantity   10 x 100 μL

Store at    -70°C.