ECOS™ Sonic™ Competent Cells [BL21(DE3) Derived]
Description
We have taken it upon ourselves to continue the production of Acella™ cells previously from Edge Biosystems Inc., which we have now renamed as ECOS™ Sonic™
Sonic™ Competent Cells allow you to clone and express in one novel strain. Also available with the pLysS plasmid, the Sonic™ strain is a BL21-derivative that features complete deletions of the endA and recA genes, eliminating degradation and recombination of DNA.
For optimal results, recommended for use in combination with Yeastern’s proprietary T&A™ expression vector, which will combine transformation, cloning and protein expression into one single step.
Genotype
F– ompT hsdsB (rB–mB–) gal dcm (DE3) ΔendA ΔrecA
Features
- Fast: Only 4-6hrs culture time required to isolates plasmid DNA
- Stable: Lack of ompT and Ion proteases to stabilize synthesized protein; deletion of endA and recA eliminates DNA degradation and recombination
- High efficiency and protein yield: 1 x 108 cfu/ug
- Simple to use: clone and express with the same strain, no more sub-cloning required!
Applications
- protein expression/ toxic protein expression
- preservation of the PCR product
- preservation of the expression plasmid
Specifications
Species E. coli
Strain BL21(DE3) Derived
Efficiency > 1 x 108 cfu/μg
(HYE007-SONIC-5VL / HYE007-SONIC-20VL)
Format Tube (100 μL)
Quantity 5 x 100 μL、20 x 100 μL
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
