ECOS™ 2163 Competent Cells [GM2163]
Description
GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes, is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes.
This strain is not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.
Genotype
F–ara–14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm–6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl–1 thi-1 mcrB1 hsdR2
Features
- Chloramphenicol resistant
- Not suitable for blue/white screening
- Not recommended as a host for primary cloning/ligation
Applications
- Propagation of plasmid-free of Dam and Dcm methylations
Species E. coli
Strain GM2163
Efficiency >1 x 107 cfu/μg
(FYE807-10VL / FYE807-80VL / FYE807-96WL)
Format Tube (100 μL) / 96-well plate (50 μL)
Quantity 10 x 100 μL、80 x 100 μL、50 μL /well
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
