ECOS™ 10B Competent Cells [DH10B]
Description
DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. The complete genome sequence has been published.
Genotype
F–endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ–
Features
- Streptomycin resistant
- High transformation efficiency
Applications
- Suitable for genomic DNA cloning
- Allow large inserts
- Cloning of methylated cytosine or adenine containing DNA
- Blue/white screening
Specifications
Species E. coli
Strain DH10B
Efficiency • > 5 x 107 cfu/μg (FYE507-10VL / FYE507-80VL )
• > 1 x 108 cfu/μg (FYE508-10VL / FYE508-80VL )
• >1 x 107 cfu/μg (FYE507-96WL)
Format Tube (100 μL) / 96-well plate (50 μL)
Quantity 10 x 100 μL、80 x 100 μL、50 μL /well
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
