DH5α is a specialized strain of Escherichia coli, engineered to possess several key features that make it an excellent choice for selective transformation. These cells have been specially treated to exhibit high efficiency in taking up foreign DNA. Here are the main features of the DH5α strain:
High transformation efficiency: DH5α cells have been processed to achieve superior transformation efficiency. This means they can effectively take up and transport foreign DNA fragments, making them ideal for cloning and genetic manipulations.
High Stability: After uptake of foreign DNA, DH5α cells can stably maintain and pass on the genetic material to their progeny. This stability is crucial for ensuring the success of genetic manipulations in research and applications.
Convenience: Given the widespread use of DH5α strain, optimal transformation and culture conditions have been established. This makes working with DH5α cells convenient and time-efficient, saving valuable resources.
Wide applications: DH5α strain finds extensive applications in cloning, mutation analysis, DNA preparation, and protein expression, among other fields. It plays a pivotal role in numerous fundamental research and applied studies.